WIRE 4
This NEG- wire is to connect the NEG- connections on your amp to the NEG- post of the rear battery. It is important that this wire matches the size (and quantity) of WIRE 2. Again, just like with WIRE 2, the size of this wire should be determined by your amps' inputs.
Some people again would tell you to not wire this to the battery, but instead to the chassis. This would be OK if you did not have a rear battery and you had a very small amp. But since this discussion is assuming the rear battery is installed, you need to wire the NEG- connection for the amp directly to the battery.