Yes. It will affect the restoring quality that the smaller box exhibits on the moving parts. What vehicle is this going in...I didn't catch that in any above posts? It's not specifically how big the box is, or where you tune it, rather the vent size and length in proportion to the box volume. As you can tell by using WinISD, there are 50,000 different port sizes you can make to tune the box to the same frequency. You want high output at low frequencies and larger enclosures do just that. The larger you go, the worse the restoring quality of the enclosure but the more port you can drive. The more port you have, the louder it is capable of getting.....in a broad perspective. Tuning that box with a bigger than "recommended" vent is what I'd recommend to get some serious output at lower frequencies...via tuning to about 32Hz with 16 square inches of vent per net cubic foot. Use WinISD to figure out the length based on your box net volume. That sized vent will add quite a bit of displacement to the box, so you'll have to play around to get the enclosure to the size you need to get it INTO the car. Nothing to do with exactly how big the spot in the car is....rather how big the opening INTO it is. If you want more low frequency extension, put the vent closer to a wall of the trunk.