personally one chamber / one port > two chambers / two ports.
one port and you know the box is tuned where you want it. with two chambers/two ports the smallest difference in port will start changing tuning freq by .00031267... etc hz. sides, greater net volume without the divide.
the only thing that changes about a box when you add a second woofer is the fact that you have to deduct the second woofer's displacement from the net volume of the box. a woofer box's tuning will change SLIGHTLY with the addition of the second driver, but probably not enough for you to notice.