Do download and figure out how to use hornresp software. EVERYBODY I have ever seen trying to build any type of horn, bin, or quarter wave box uses it.
You can safely say you'll lose an inch from the diameter of the driver for surround, that should get you close enough. Speaking of close enough if you're off on an end 3hz change in tuning of a box is inaudible in my experience and even 5hz is barely audible so don't drive yourself crazy there.
According to that source the unwanted high resonances that you think you want to tame with stuffing are only an issue in full range system so you should not need any batting unless you're trying to cheat a shorter line and from my experience with stuffing in general while you can change response slightly you pay for it with efficiency.
I do not believe you need a chamber for a true transmission line, that becomes more of a horn or just a ported box. AFAIK true T-line is the woofer firing into the line which is consistent length throughout. Having an odd size chamber puts you more into horn territory, though I suspect the hornresp software will have options where you can play with that.
Lastly I think if you want to play down to 20hz you will not be able to build this without a bend. You are getting into a LONG wavelength, and that said I'm not sure how far below FS you can go with a t-line. There have only been a handful of guys on this forum who have built these so if you want better, do a forum search and see who has done them, PM those guys and hope they're still active. Good luck and please post a build log as you go. I've heard one proper T line in person and it sounded very nice. Almost worth the space and the wood. Mind you, with today's EQ options and how cheap power is and how robust our woofers are there's no reason you can't get flat from 20-80hz in the same space a few different ways these days. I'd consider T-line not something you "have to have" in order to get a particular sound but just an alternative as something to do if you like a good DIY woodworking challenge and interesting conversation piece.
Good luck and please post a build log here.