The resin you buy will have instructions on how much MEKP to mix with the resin. You want to mix relatively small amounts at a time, because if you take too long the resin will 'gel' up and become useless. Also, note that the mixtures listed are for 70degrees F. If it is warmer, you can use slightly less, if colder use more. If you use too little, it will never harden -- to much and your work time will be cut to less than 5 minute
You can lay a second layer of fiberglass right on top of the first one. Although I would not go more than 3 layers thick before waiting for it to completely dry, then restarting the process. When you're done layering, clean your tools and wait. If you mixed you mixture right, it should be dry to the touch in about 1.5 - 2 hours. You can then lay more layers as needed. For this small box, only 2 layers were used. For larger fiberglass boxes I usually use between 7 - 9 layers, thicker if there are any flat runs that i couldnt make out of wood. Also if you use a thicker matt, you obvisouly can use less layers. Keep in mind, however, that a thick matt will not conform to tight corners very well.