It really depends on what subs you are running, and how much power you have for them. I heard most say to use thicker on the plexi than the MDF. If you were going to make a box out of 1/2 MDF, then use 3/4 plexi. Or if the box was 3/4 MDF then use 1" plexi. This is only what I have heard, but the cost of 1" plexi was too much. So back to the mdf I went.