THE Thread

Drosophila melanogaster, or the red-eyed pomace fly, is classified in the family Drosophilidae, and order Diptera (which also includes flies, mosquitoes and midges.)

In the wild, they are found near ripe fruit where the adults and larvae feed on yeast and bacteria growing on rotting fruit. In the lab, they can be fed on yeast cells growing on a high carbohydrate prepared diet.

The average life span of Drosophila depends on the environmental conditions. There are records of 80-153 days, but the average life span of a Lab fly is 26 days for a female, and 33 days for a male. (Under crowded conditions this may be reduced to 12 days. Also mutant flies generally have a shorter life span). Temperature greatly effects the rate of development. At room temperature (25˚C): 10 days from egg to adult; at 20 ˚C: 13 days; at 15˚C: 90 days. There are four phases to the life cycle: egg - 3 larval (instar)stages - pupa - adult.

Courtship begins by the male tapping the abdomen of the female with his foreleg. This is his means of identifying his own species. He approaches the female fly from the front, and circles around her making half turns. He sticks a wing out, vibrating it for several seconds. If the female is receptive, copulation results. Sperm from the male are held in the seminal receptacle or spermatheca. There may be more than one mating. Eggs are .5 mm long, and are laid generally after the third day of the female’s adult life.

Larvae hatch in 22 hours, and grow and feed for four days, (longer at lower temperatures). The larvae are transparent, and one can see the inside organs such as the coiled intestines, whitish fat bodies, and gonads (visible in the male but very small in the female). During the third instar stage of larval development, observers of Drosophila will notice the larvae crawling up the sides of the culture container in preparation for pupation. At this time one can see dark projections called pupal horns, off the anterior end. These are the spiracles (outside opening of the respiratory tubes) turned inside out.

The pupal case forms, darkens and hardens. Sexing of the pupae can be done by looking for tarsal *** combs of the male, visible from the ventral side. The *** combs in the adult males, are tufts of dark hair found on the most proximal tarsal joint of the foreleg, used for holding the female during mating. Even in the pupal phase, these dark patches are visible in males.

The pupal stage lasts for 4 - 6 days, during which time metamorphosis occurs. Larval tissues are broken down (except for the brain and a few other tissues), and imaginal discs (pockets of cells stored in the larvae) develop into adult organs. There is a disc for each leg, wing, eye, antennae etc. Finally the pupa is ready to eclose (emerge) into the adult stage. Adult Drosophila males and females can be easily distinguished. Males are smaller, with a rounded, blackened tip to their abdomen (posterior segment). Females have a pointed abdomen, with a pattern of even dark bands.

 
Activity
No one is currently typing a reply...

About this thread

RangerMan

Premium Member
Premium Member
Thread starter
RangerMan
Joined
Location
NC
Start date
Participants
Who Replied
Replies
182,982
Views
3,295,726
Last reply date
Last reply from
x2o
IMG_20260506_140749.jpg

74eldiablo

    May 22, 2026
  • 0
  • 0
design.jpeg

WNCTracker

    May 22, 2026
  • 0
  • 0

New threads

Top